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murine recombinant thpo  (PeproTech)


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    PeproTech murine recombinant thpo
    Murine Recombinant Thpo, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+recombinant+thpo/recombinant+human+thpo/pm30428347-248-164-166
    Average 90 stars, based on 1 article reviews
    murine recombinant thpo - by Bioz Stars, 2026-10
    90/100 stars

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    Recombinant:

    Article Title: Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD16/32 [93] eBioscience / Biolegend 14-0161 / 101320; RRID: AB_312800/AB_467132 CD16/32 [93], AF700 eBioscience 56-0161-82; RRID: AB_493994 CD4 [RM4-5], PerCp-Cy5.5 Biolegend 100540; RRID: AB_2563022 CD8a [53-6.7], PerCp-Cy5.5 Biolegend 100734; RRID: AB_893427 Gr-1 [RB6-8C5], PerCp-Cy5.5 Biolegend 108428; RRID: AB_893561 Mac-1 [M1/70], PerCp-Cy5.5 Biolegend 101228; RRID: AB_893233 B220 [RA3-6B2], PerCp-Cy5.5 Biolegend 103236; RRID: AB_893356 Ter-119 [TER-119], PerCp-Cy5.5 Biolegend 116228; RRID: AB_893638 c-Kit [2B8], APC-Cy7 Biolegend 105826; RRID: AB_1626280 Sca-1 [D7], PE-Cy7 Biolegend 108114; RIID: AB_493597 Sca-1 [D7], APC-Cy7 Biolegend 108126; RRID: AB_10639725 CD150 [TC15-12F12.2], APC Biolegend 115910; RRID: AB_493461 CD150 [TC15-12F12.2], PE Biolegend 115904; RRID: AB_313682 CD150 [TC15-12F12.2], PEcy7 Biolegend 115914; RRID: AB_439796 CD48 [HM48-1], AF700 Biolegend 103426; RRID: AB_10612754 CD45.1 [A20], PE Biolegend 110708; RRID: AB_313496 CD45.1 [A20], AF700 Biolegend 110724; RRID: AB_493732 CD45.2 [104], APC-Cy7 Biolegend 109824; RRID: AB_830788 CD8a [53-6.7], PE-Cy7 Biolegend 100722; RRID: AB_312760 Gr-1 [RB6-8C5], APC BD 553129; RRID: AB_2314614 Mac-1 [M1/70], APC Biolegend 101212; RRID: AB_312794 B220 [RA3-6B2], PECy7 BD 552772; RRID: AB_394458 c-Kit [2B8], BV421 Biolegend 105828; RRID: AB_10898120 Flt-3 [A2F10.1], APC eBioscience 17-1351-80; RRID: AB_10596653 Flt-3 [A2F10.1], PE Biolegend 135305; RRID: AB_1877217 CD41 [MWReg30], PE BD 558040; RRID: AB_397004 CD41 [MWReg30], APC Invitrogen 17-0411-82; RRID: AB_1603238 IL-7Ra [A7R34], PE eBioscience 12-1271-81; RRID: AB_465845 IL-7Ra [A7R34], BV510 Biolegend 135033; RRID: AB_2564576 Endoglin [MJ7/18] Biolegend 120410; RRID: AB_1027702 CD9 [KMC8], BV421 BD Horizon 564235; RRID: AB_395032 CD34 [RAM34], FITC eBioscience 11-034-85; RRID: AB_465020 Mpl [AMM2], Biotin IBL 10403 Ki67 [SolA15], PE eBioscience 12-5698-82; RRID: AB_11150954 AnnexinV, PE Life Technologies A35111 pSTAT3 (S727)[49] BD Phosflow 558557 GRIM19 [6E1BH7] Abcam ab110240; RRID: AB_303811 TOMM20 Abcam Ab186734; RRID: AB_2043078 pAMPKa (Thr172) [40H9] CST 2535; RRID: AB_331250 CD41-143Nd [MWreg30] Fluidigm 3143009B SOCS3 [516919] R&D MAB5696; RRID: AB_2193299 pStat5 (Y694)-147Sm [47] Fluidigm 3147012A SOCS1 [4H1] ThermoScientific 37-4100; RRID: AB_10890785 (Continued on next page) e1 Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER anti-biotin-150Nd Fluidigm 3150008B pAkt (S473) 152Sm [D9E] Fluidigm 3152005A pStat1 (Y701)- 153Eu [58D6] Fluidigm 3153003A CD48-154Sm [HM48-1] Fluidigm 3154004B p38 (T180/Y182)- 156Gd [D3F9] Fluidigm 3156002A pStat3 (Y705)- 158Gd [4] Fluidigm 3158005A pMAPKAPK2 (T334)- 159Tb [27B7] Fluidigm 3159010A pJak2 (Tyr1008) [D4A8] CST 8082S; RRID: AB_10949104 Tie2 [TEK4] Biolegend 124002; RRID: AB_961175 mTOR (phospho S2448) [EPR426(2)] Abcam ab109268; RRID: AB_297588 EPCR [eBio1560] eBioscience 16-2012-83; RRID: AB_657692 CD150 167Er [TC15-12F12.2] Fluidigm 3167004B CD61 [2C9.G2] Biolegend 104302; RRID: AB_313079 Ly-6A/E (Sca-1)- 169Tm [D7] Fluidigm 3169015B pERK 1/2 (T202/Y204)- 171Yb [D13.14.4E] Fluidigm 3171010A CD117 (ckit)- 173Yb [2B8] Fluidigm 3173004B pStat3 (S727) [ab30647] Abcam ab86430; RRID: AB_779085 Pgc1a Abcam ab54481; RRID: AB_881987 Chemicals, Peptides, and Recombinant Proteins Romiplostim Kyowa Kirin n/a MitotrackerTM Green FM ThermoScientific M7514 TMRE Enzo 52309 CellROX ThermoScientific C10448 recombinant human Thpo (PEG-rHuMGDF) Kyowa Kirin n/a Filgrastim Kyowa Kirin n/a antimycin Sigma A8674 rotenone Abcam ab143145 oligomycin Abcam ab141829 FCCP Abcam ab120081 KPT-330 Cayman chemical 18127 murine recombinant SCF Peprotech 250-03 murine recombinant Thpo Peprotech 315-14 cisplatin (Cell-ID Cisplatin) Fluidigm 201198 Critical Commercial Assays CellTiter-Glo 2.0 Assay kit Promega G9241 MegaCult Stem cell Technologies 04960 MethoCult Stem cell Technologies M3234 LIVE/DEAD Viability/cytotoxicity Kit Life Technologies MP03224 SMARTer Ultra Low RNA Kit for Fluidigm C1 system Clonetech 634936 Illumina Nextera XT DNA Sample Preparation Kit Illumina FC-131-1096/FC-131-1002 C1 Single-cell Auto Prep Reagent kit for mRNA-seq Fluidigm 100-6201 Superscript VILO Invitrogen 11754250 RNeasy Mini Kit QIAGEN 74106 IntraPrep reagents Beckman Coulter IM2389 Maxpar antibody labeling kit Fluidigm 201300 MaxPar Fix I buffer Fluidigm 201065 Maxpar Cell Staining Buffer Fluidigm 201068 Cell-ID Intercalator-Ir Fluidigm 201192A Maxpar Fix and Perm Buffer Fluidigm 201057 (Continued on next page) Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data RNA-seq: Thrombopoietin metabolically primes hematopoietic stem cells to megakaryocyte lineage differentiation This paper GEO: GSE121001 Experimental Models: Organisms/Strains Mouse: Thpo / Toshio Suda Lab n/a Mouse: UBC-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J) Jackson laboratory 004353 Mouse: CD45.1 Jackson laboratory 002014 Mouse: Mito-Dendra2 (Gt(ROSA)26Sortm1.1(CAG-COX8A/ Dendra2)Dcc) Jackson laboratory 018385 Mouse: (Fucci) GMNN-mAG mice (B6.Cg-Tg(FucciS/G2/M)#474Bsi RIKEN RBRC02704 Oligonucleotides Taqman primer: Ppargc1a Thermo Fisher Scientific Mm01208835_m1 Taqman primer: Tfam Thermo Fisher Scientific Mm00447485_m1 Taqman primer: Nrf1 Thermo Fisher Scientific Mm01135606_m1 Taqman primer: Sdhb Thermo Fisher Scientific Mm00458272_m1 Taqman primer: Sdhc Thermo Fisher Scientific Mm00481172_m1 Taqman primer: Atp5a Thermo Fisher Scientific Mm00431960_m1 Taqman primer: Cox5a Thermo Fisher Scientific Mm00432638_m1 Taqman primer: Cyc1 Thermo Fisher Scientific Mm00470540_m1 Taqman primer: Ndufa13 Thermo Fisher Scientific Mm00445751_m1 Taqman primer: B2m Thermo Fisher Scientific Mm03003532_u1 Taqman primer: Bax Thermo Fisher Scientific Mm00432051_m1 Taqman primer: Bak1 Thermo Fisher Scientific Mm00432045_m1 Taqman primer: Bcl2 Thermo Fisher Scientific Mm00477631_m1 Taqman primer: Prdx1 Thermo Fisher Scientific Mm01621996_s1 Taqman primer: Prdx3 Thermo Fisher Scientific Mm00545848_m1 Taqman primer: Gpx1 Thermo Fisher Scientific Mm00656767_g1 Taqman primer: Gpx4 Thermo Fisher Scientific Mm00515041_m1 Taqman primer: Txn2 Thermo Fisher Scientific Mm00444931_m1 Software and Algorithms FlowJo 10.1 Tree Star n/a QuantStudioTM Design and Analysis Software 1.3.1 Thermo Fisher Scientific n/a Agilent Seahorse Wave Desktop Agilent n/a R R Foundation version 3.3.2 Rtsne package version 0.13 Cufflinks version 2.2.1 PANTHER release 20160715 GSEA Broad institute Version 2.0.13 NIS elements Nikon n/a ImageJ n/a Cytobank. n/a flowCore n/a flowViz n/a flowUtils n/a

    Sample Prep:

    Article Title: Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD16/32 [93] eBioscience / Biolegend 14-0161 / 101320; RRID: AB_312800/AB_467132 CD16/32 [93], AF700 eBioscience 56-0161-82; RRID: AB_493994 CD4 [RM4-5], PerCp-Cy5.5 Biolegend 100540; RRID: AB_2563022 CD8a [53-6.7], PerCp-Cy5.5 Biolegend 100734; RRID: AB_893427 Gr-1 [RB6-8C5], PerCp-Cy5.5 Biolegend 108428; RRID: AB_893561 Mac-1 [M1/70], PerCp-Cy5.5 Biolegend 101228; RRID: AB_893233 B220 [RA3-6B2], PerCp-Cy5.5 Biolegend 103236; RRID: AB_893356 Ter-119 [TER-119], PerCp-Cy5.5 Biolegend 116228; RRID: AB_893638 c-Kit [2B8], APC-Cy7 Biolegend 105826; RRID: AB_1626280 Sca-1 [D7], PE-Cy7 Biolegend 108114; RIID: AB_493597 Sca-1 [D7], APC-Cy7 Biolegend 108126; RRID: AB_10639725 CD150 [TC15-12F12.2], APC Biolegend 115910; RRID: AB_493461 CD150 [TC15-12F12.2], PE Biolegend 115904; RRID: AB_313682 CD150 [TC15-12F12.2], PEcy7 Biolegend 115914; RRID: AB_439796 CD48 [HM48-1], AF700 Biolegend 103426; RRID: AB_10612754 CD45.1 [A20], PE Biolegend 110708; RRID: AB_313496 CD45.1 [A20], AF700 Biolegend 110724; RRID: AB_493732 CD45.2 [104], APC-Cy7 Biolegend 109824; RRID: AB_830788 CD8a [53-6.7], PE-Cy7 Biolegend 100722; RRID: AB_312760 Gr-1 [RB6-8C5], APC BD 553129; RRID: AB_2314614 Mac-1 [M1/70], APC Biolegend 101212; RRID: AB_312794 B220 [RA3-6B2], PECy7 BD 552772; RRID: AB_394458 c-Kit [2B8], BV421 Biolegend 105828; RRID: AB_10898120 Flt-3 [A2F10.1], APC eBioscience 17-1351-80; RRID: AB_10596653 Flt-3 [A2F10.1], PE Biolegend 135305; RRID: AB_1877217 CD41 [MWReg30], PE BD 558040; RRID: AB_397004 CD41 [MWReg30], APC Invitrogen 17-0411-82; RRID: AB_1603238 IL-7Ra [A7R34], PE eBioscience 12-1271-81; RRID: AB_465845 IL-7Ra [A7R34], BV510 Biolegend 135033; RRID: AB_2564576 Endoglin [MJ7/18] Biolegend 120410; RRID: AB_1027702 CD9 [KMC8], BV421 BD Horizon 564235; RRID: AB_395032 CD34 [RAM34], FITC eBioscience 11-034-85; RRID: AB_465020 Mpl [AMM2], Biotin IBL 10403 Ki67 [SolA15], PE eBioscience 12-5698-82; RRID: AB_11150954 AnnexinV, PE Life Technologies A35111 pSTAT3 (S727)[49] BD Phosflow 558557 GRIM19 [6E1BH7] Abcam ab110240; RRID: AB_303811 TOMM20 Abcam Ab186734; RRID: AB_2043078 pAMPKa (Thr172) [40H9] CST 2535; RRID: AB_331250 CD41-143Nd [MWreg30] Fluidigm 3143009B SOCS3 [516919] R&D MAB5696; RRID: AB_2193299 pStat5 (Y694)-147Sm [47] Fluidigm 3147012A SOCS1 [4H1] ThermoScientific 37-4100; RRID: AB_10890785 (Continued on next page) e1 Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER anti-biotin-150Nd Fluidigm 3150008B pAkt (S473) 152Sm [D9E] Fluidigm 3152005A pStat1 (Y701)- 153Eu [58D6] Fluidigm 3153003A CD48-154Sm [HM48-1] Fluidigm 3154004B p38 (T180/Y182)- 156Gd [D3F9] Fluidigm 3156002A pStat3 (Y705)- 158Gd [4] Fluidigm 3158005A pMAPKAPK2 (T334)- 159Tb [27B7] Fluidigm 3159010A pJak2 (Tyr1008) [D4A8] CST 8082S; RRID: AB_10949104 Tie2 [TEK4] Biolegend 124002; RRID: AB_961175 mTOR (phospho S2448) [EPR426(2)] Abcam ab109268; RRID: AB_297588 EPCR [eBio1560] eBioscience 16-2012-83; RRID: AB_657692 CD150 167Er [TC15-12F12.2] Fluidigm 3167004B CD61 [2C9.G2] Biolegend 104302; RRID: AB_313079 Ly-6A/E (Sca-1)- 169Tm [D7] Fluidigm 3169015B pERK 1/2 (T202/Y204)- 171Yb [D13.14.4E] Fluidigm 3171010A CD117 (ckit)- 173Yb [2B8] Fluidigm 3173004B pStat3 (S727) [ab30647] Abcam ab86430; RRID: AB_779085 Pgc1a Abcam ab54481; RRID: AB_881987 Chemicals, Peptides, and Recombinant Proteins Romiplostim Kyowa Kirin n/a MitotrackerTM Green FM ThermoScientific M7514 TMRE Enzo 52309 CellROX ThermoScientific C10448 recombinant human Thpo (PEG-rHuMGDF) Kyowa Kirin n/a Filgrastim Kyowa Kirin n/a antimycin Sigma A8674 rotenone Abcam ab143145 oligomycin Abcam ab141829 FCCP Abcam ab120081 KPT-330 Cayman chemical 18127 murine recombinant SCF Peprotech 250-03 murine recombinant Thpo Peprotech 315-14 cisplatin (Cell-ID Cisplatin) Fluidigm 201198 Critical Commercial Assays CellTiter-Glo 2.0 Assay kit Promega G9241 MegaCult Stem cell Technologies 04960 MethoCult Stem cell Technologies M3234 LIVE/DEAD Viability/cytotoxicity Kit Life Technologies MP03224 SMARTer Ultra Low RNA Kit for Fluidigm C1 system Clonetech 634936 Illumina Nextera XT DNA Sample Preparation Kit Illumina FC-131-1096/FC-131-1002 C1 Single-cell Auto Prep Reagent kit for mRNA-seq Fluidigm 100-6201 Superscript VILO Invitrogen 11754250 RNeasy Mini Kit QIAGEN 74106 IntraPrep reagents Beckman Coulter IM2389 Maxpar antibody labeling kit Fluidigm 201300 MaxPar Fix I buffer Fluidigm 201065 Maxpar Cell Staining Buffer Fluidigm 201068 Cell-ID Intercalator-Ir Fluidigm 201192A Maxpar Fix and Perm Buffer Fluidigm 201057 (Continued on next page) Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data RNA-seq: Thrombopoietin metabolically primes hematopoietic stem cells to megakaryocyte lineage differentiation This paper GEO: GSE121001 Experimental Models: Organisms/Strains Mouse: Thpo / Toshio Suda Lab n/a Mouse: UBC-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J) Jackson laboratory 004353 Mouse: CD45.1 Jackson laboratory 002014 Mouse: Mito-Dendra2 (Gt(ROSA)26Sortm1.1(CAG-COX8A/ Dendra2)Dcc) Jackson laboratory 018385 Mouse: (Fucci) GMNN-mAG mice (B6.Cg-Tg(FucciS/G2/M)#474Bsi RIKEN RBRC02704 Oligonucleotides Taqman primer: Ppargc1a Thermo Fisher Scientific Mm01208835_m1 Taqman primer: Tfam Thermo Fisher Scientific Mm00447485_m1 Taqman primer: Nrf1 Thermo Fisher Scientific Mm01135606_m1 Taqman primer: Sdhb Thermo Fisher Scientific Mm00458272_m1 Taqman primer: Sdhc Thermo Fisher Scientific Mm00481172_m1 Taqman primer: Atp5a Thermo Fisher Scientific Mm00431960_m1 Taqman primer: Cox5a Thermo Fisher Scientific Mm00432638_m1 Taqman primer: Cyc1 Thermo Fisher Scientific Mm00470540_m1 Taqman primer: Ndufa13 Thermo Fisher Scientific Mm00445751_m1 Taqman primer: B2m Thermo Fisher Scientific Mm03003532_u1 Taqman primer: Bax Thermo Fisher Scientific Mm00432051_m1 Taqman primer: Bak1 Thermo Fisher Scientific Mm00432045_m1 Taqman primer: Bcl2 Thermo Fisher Scientific Mm00477631_m1 Taqman primer: Prdx1 Thermo Fisher Scientific Mm01621996_s1 Taqman primer: Prdx3 Thermo Fisher Scientific Mm00545848_m1 Taqman primer: Gpx1 Thermo Fisher Scientific Mm00656767_g1 Taqman primer: Gpx4 Thermo Fisher Scientific Mm00515041_m1 Taqman primer: Txn2 Thermo Fisher Scientific Mm00444931_m1 Software and Algorithms FlowJo 10.1 Tree Star n/a QuantStudioTM Design and Analysis Software 1.3.1 Thermo Fisher Scientific n/a Agilent Seahorse Wave Desktop Agilent n/a R R Foundation version 3.3.2 Rtsne package version 0.13 Cufflinks version 2.2.1 PANTHER release 20160715 GSEA Broad institute Version 2.0.13 NIS elements Nikon n/a ImageJ n/a Cytobank. n/a flowCore n/a flowViz n/a flowUtils n/a

    Single Cell:

    Article Title: Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD16/32 [93] eBioscience / Biolegend 14-0161 / 101320; RRID: AB_312800/AB_467132 CD16/32 [93], AF700 eBioscience 56-0161-82; RRID: AB_493994 CD4 [RM4-5], PerCp-Cy5.5 Biolegend 100540; RRID: AB_2563022 CD8a [53-6.7], PerCp-Cy5.5 Biolegend 100734; RRID: AB_893427 Gr-1 [RB6-8C5], PerCp-Cy5.5 Biolegend 108428; RRID: AB_893561 Mac-1 [M1/70], PerCp-Cy5.5 Biolegend 101228; RRID: AB_893233 B220 [RA3-6B2], PerCp-Cy5.5 Biolegend 103236; RRID: AB_893356 Ter-119 [TER-119], PerCp-Cy5.5 Biolegend 116228; RRID: AB_893638 c-Kit [2B8], APC-Cy7 Biolegend 105826; RRID: AB_1626280 Sca-1 [D7], PE-Cy7 Biolegend 108114; RIID: AB_493597 Sca-1 [D7], APC-Cy7 Biolegend 108126; RRID: AB_10639725 CD150 [TC15-12F12.2], APC Biolegend 115910; RRID: AB_493461 CD150 [TC15-12F12.2], PE Biolegend 115904; RRID: AB_313682 CD150 [TC15-12F12.2], PEcy7 Biolegend 115914; RRID: AB_439796 CD48 [HM48-1], AF700 Biolegend 103426; RRID: AB_10612754 CD45.1 [A20], PE Biolegend 110708; RRID: AB_313496 CD45.1 [A20], AF700 Biolegend 110724; RRID: AB_493732 CD45.2 [104], APC-Cy7 Biolegend 109824; RRID: AB_830788 CD8a [53-6.7], PE-Cy7 Biolegend 100722; RRID: AB_312760 Gr-1 [RB6-8C5], APC BD 553129; RRID: AB_2314614 Mac-1 [M1/70], APC Biolegend 101212; RRID: AB_312794 B220 [RA3-6B2], PECy7 BD 552772; RRID: AB_394458 c-Kit [2B8], BV421 Biolegend 105828; RRID: AB_10898120 Flt-3 [A2F10.1], APC eBioscience 17-1351-80; RRID: AB_10596653 Flt-3 [A2F10.1], PE Biolegend 135305; RRID: AB_1877217 CD41 [MWReg30], PE BD 558040; RRID: AB_397004 CD41 [MWReg30], APC Invitrogen 17-0411-82; RRID: AB_1603238 IL-7Ra [A7R34], PE eBioscience 12-1271-81; RRID: AB_465845 IL-7Ra [A7R34], BV510 Biolegend 135033; RRID: AB_2564576 Endoglin [MJ7/18] Biolegend 120410; RRID: AB_1027702 CD9 [KMC8], BV421 BD Horizon 564235; RRID: AB_395032 CD34 [RAM34], FITC eBioscience 11-034-85; RRID: AB_465020 Mpl [AMM2], Biotin IBL 10403 Ki67 [SolA15], PE eBioscience 12-5698-82; RRID: AB_11150954 AnnexinV, PE Life Technologies A35111 pSTAT3 (S727)[49] BD Phosflow 558557 GRIM19 [6E1BH7] Abcam ab110240; RRID: AB_303811 TOMM20 Abcam Ab186734; RRID: AB_2043078 pAMPKa (Thr172) [40H9] CST 2535; RRID: AB_331250 CD41-143Nd [MWreg30] Fluidigm 3143009B SOCS3 [516919] R&D MAB5696; RRID: AB_2193299 pStat5 (Y694)-147Sm [47] Fluidigm 3147012A SOCS1 [4H1] ThermoScientific 37-4100; RRID: AB_10890785 (Continued on next page) e1 Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER anti-biotin-150Nd Fluidigm 3150008B pAkt (S473) 152Sm [D9E] Fluidigm 3152005A pStat1 (Y701)- 153Eu [58D6] Fluidigm 3153003A CD48-154Sm [HM48-1] Fluidigm 3154004B p38 (T180/Y182)- 156Gd [D3F9] Fluidigm 3156002A pStat3 (Y705)- 158Gd [4] Fluidigm 3158005A pMAPKAPK2 (T334)- 159Tb [27B7] Fluidigm 3159010A pJak2 (Tyr1008) [D4A8] CST 8082S; RRID: AB_10949104 Tie2 [TEK4] Biolegend 124002; RRID: AB_961175 mTOR (phospho S2448) [EPR426(2)] Abcam ab109268; RRID: AB_297588 EPCR [eBio1560] eBioscience 16-2012-83; RRID: AB_657692 CD150 167Er [TC15-12F12.2] Fluidigm 3167004B CD61 [2C9.G2] Biolegend 104302; RRID: AB_313079 Ly-6A/E (Sca-1)- 169Tm [D7] Fluidigm 3169015B pERK 1/2 (T202/Y204)- 171Yb [D13.14.4E] Fluidigm 3171010A CD117 (ckit)- 173Yb [2B8] Fluidigm 3173004B pStat3 (S727) [ab30647] Abcam ab86430; RRID: AB_779085 Pgc1a Abcam ab54481; RRID: AB_881987 Chemicals, Peptides, and Recombinant Proteins Romiplostim Kyowa Kirin n/a MitotrackerTM Green FM ThermoScientific M7514 TMRE Enzo 52309 CellROX ThermoScientific C10448 recombinant human Thpo (PEG-rHuMGDF) Kyowa Kirin n/a Filgrastim Kyowa Kirin n/a antimycin Sigma A8674 rotenone Abcam ab143145 oligomycin Abcam ab141829 FCCP Abcam ab120081 KPT-330 Cayman chemical 18127 murine recombinant SCF Peprotech 250-03 murine recombinant Thpo Peprotech 315-14 cisplatin (Cell-ID Cisplatin) Fluidigm 201198 Critical Commercial Assays CellTiter-Glo 2.0 Assay kit Promega G9241 MegaCult Stem cell Technologies 04960 MethoCult Stem cell Technologies M3234 LIVE/DEAD Viability/cytotoxicity Kit Life Technologies MP03224 SMARTer Ultra Low RNA Kit for Fluidigm C1 system Clonetech 634936 Illumina Nextera XT DNA Sample Preparation Kit Illumina FC-131-1096/FC-131-1002 C1 Single-cell Auto Prep Reagent kit for mRNA-seq Fluidigm 100-6201 Superscript VILO Invitrogen 11754250 RNeasy Mini Kit QIAGEN 74106 IntraPrep reagents Beckman Coulter IM2389 Maxpar antibody labeling kit Fluidigm 201300 MaxPar Fix I buffer Fluidigm 201065 Maxpar Cell Staining Buffer Fluidigm 201068 Cell-ID Intercalator-Ir Fluidigm 201192A Maxpar Fix and Perm Buffer Fluidigm 201057 (Continued on next page) Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data RNA-seq: Thrombopoietin metabolically primes hematopoietic stem cells to megakaryocyte lineage differentiation This paper GEO: GSE121001 Experimental Models: Organisms/Strains Mouse: Thpo / Toshio Suda Lab n/a Mouse: UBC-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J) Jackson laboratory 004353 Mouse: CD45.1 Jackson laboratory 002014 Mouse: Mito-Dendra2 (Gt(ROSA)26Sortm1.1(CAG-COX8A/ Dendra2)Dcc) Jackson laboratory 018385 Mouse: (Fucci) GMNN-mAG mice (B6.Cg-Tg(FucciS/G2/M)#474Bsi RIKEN RBRC02704 Oligonucleotides Taqman primer: Ppargc1a Thermo Fisher Scientific Mm01208835_m1 Taqman primer: Tfam Thermo Fisher Scientific Mm00447485_m1 Taqman primer: Nrf1 Thermo Fisher Scientific Mm01135606_m1 Taqman primer: Sdhb Thermo Fisher Scientific Mm00458272_m1 Taqman primer: Sdhc Thermo Fisher Scientific Mm00481172_m1 Taqman primer: Atp5a Thermo Fisher Scientific Mm00431960_m1 Taqman primer: Cox5a Thermo Fisher Scientific Mm00432638_m1 Taqman primer: Cyc1 Thermo Fisher Scientific Mm00470540_m1 Taqman primer: Ndufa13 Thermo Fisher Scientific Mm00445751_m1 Taqman primer: B2m Thermo Fisher Scientific Mm03003532_u1 Taqman primer: Bax Thermo Fisher Scientific Mm00432051_m1 Taqman primer: Bak1 Thermo Fisher Scientific Mm00432045_m1 Taqman primer: Bcl2 Thermo Fisher Scientific Mm00477631_m1 Taqman primer: Prdx1 Thermo Fisher Scientific Mm01621996_s1 Taqman primer: Prdx3 Thermo Fisher Scientific Mm00545848_m1 Taqman primer: Gpx1 Thermo Fisher Scientific Mm00656767_g1 Taqman primer: Gpx4 Thermo Fisher Scientific Mm00515041_m1 Taqman primer: Txn2 Thermo Fisher Scientific Mm00444931_m1 Software and Algorithms FlowJo 10.1 Tree Star n/a QuantStudioTM Design and Analysis Software 1.3.1 Thermo Fisher Scientific n/a Agilent Seahorse Wave Desktop Agilent n/a R R Foundation version 3.3.2 Rtsne package version 0.13 Cufflinks version 2.2.1 PANTHER release 20160715 GSEA Broad institute Version 2.0.13 NIS elements Nikon n/a ImageJ n/a Cytobank. n/a flowCore n/a flowViz n/a flowUtils n/a

    Antibody Labeling:

    Article Title: Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD16/32 [93] eBioscience / Biolegend 14-0161 / 101320; RRID: AB_312800/AB_467132 CD16/32 [93], AF700 eBioscience 56-0161-82; RRID: AB_493994 CD4 [RM4-5], PerCp-Cy5.5 Biolegend 100540; RRID: AB_2563022 CD8a [53-6.7], PerCp-Cy5.5 Biolegend 100734; RRID: AB_893427 Gr-1 [RB6-8C5], PerCp-Cy5.5 Biolegend 108428; RRID: AB_893561 Mac-1 [M1/70], PerCp-Cy5.5 Biolegend 101228; RRID: AB_893233 B220 [RA3-6B2], PerCp-Cy5.5 Biolegend 103236; RRID: AB_893356 Ter-119 [TER-119], PerCp-Cy5.5 Biolegend 116228; RRID: AB_893638 c-Kit [2B8], APC-Cy7 Biolegend 105826; RRID: AB_1626280 Sca-1 [D7], PE-Cy7 Biolegend 108114; RIID: AB_493597 Sca-1 [D7], APC-Cy7 Biolegend 108126; RRID: AB_10639725 CD150 [TC15-12F12.2], APC Biolegend 115910; RRID: AB_493461 CD150 [TC15-12F12.2], PE Biolegend 115904; RRID: AB_313682 CD150 [TC15-12F12.2], PEcy7 Biolegend 115914; RRID: AB_439796 CD48 [HM48-1], AF700 Biolegend 103426; RRID: AB_10612754 CD45.1 [A20], PE Biolegend 110708; RRID: AB_313496 CD45.1 [A20], AF700 Biolegend 110724; RRID: AB_493732 CD45.2 [104], APC-Cy7 Biolegend 109824; RRID: AB_830788 CD8a [53-6.7], PE-Cy7 Biolegend 100722; RRID: AB_312760 Gr-1 [RB6-8C5], APC BD 553129; RRID: AB_2314614 Mac-1 [M1/70], APC Biolegend 101212; RRID: AB_312794 B220 [RA3-6B2], PECy7 BD 552772; RRID: AB_394458 c-Kit [2B8], BV421 Biolegend 105828; RRID: AB_10898120 Flt-3 [A2F10.1], APC eBioscience 17-1351-80; RRID: AB_10596653 Flt-3 [A2F10.1], PE Biolegend 135305; RRID: AB_1877217 CD41 [MWReg30], PE BD 558040; RRID: AB_397004 CD41 [MWReg30], APC Invitrogen 17-0411-82; RRID: AB_1603238 IL-7Ra [A7R34], PE eBioscience 12-1271-81; RRID: AB_465845 IL-7Ra [A7R34], BV510 Biolegend 135033; RRID: AB_2564576 Endoglin [MJ7/18] Biolegend 120410; RRID: AB_1027702 CD9 [KMC8], BV421 BD Horizon 564235; RRID: AB_395032 CD34 [RAM34], FITC eBioscience 11-034-85; RRID: AB_465020 Mpl [AMM2], Biotin IBL 10403 Ki67 [SolA15], PE eBioscience 12-5698-82; RRID: AB_11150954 AnnexinV, PE Life Technologies A35111 pSTAT3 (S727)[49] BD Phosflow 558557 GRIM19 [6E1BH7] Abcam ab110240; RRID: AB_303811 TOMM20 Abcam Ab186734; RRID: AB_2043078 pAMPKa (Thr172) [40H9] CST 2535; RRID: AB_331250 CD41-143Nd [MWreg30] Fluidigm 3143009B SOCS3 [516919] R&D MAB5696; RRID: AB_2193299 pStat5 (Y694)-147Sm [47] Fluidigm 3147012A SOCS1 [4H1] ThermoScientific 37-4100; RRID: AB_10890785 (Continued on next page) e1 Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER anti-biotin-150Nd Fluidigm 3150008B pAkt (S473) 152Sm [D9E] Fluidigm 3152005A pStat1 (Y701)- 153Eu [58D6] Fluidigm 3153003A CD48-154Sm [HM48-1] Fluidigm 3154004B p38 (T180/Y182)- 156Gd [D3F9] Fluidigm 3156002A pStat3 (Y705)- 158Gd [4] Fluidigm 3158005A pMAPKAPK2 (T334)- 159Tb [27B7] Fluidigm 3159010A pJak2 (Tyr1008) [D4A8] CST 8082S; RRID: AB_10949104 Tie2 [TEK4] Biolegend 124002; RRID: AB_961175 mTOR (phospho S2448) [EPR426(2)] Abcam ab109268; RRID: AB_297588 EPCR [eBio1560] eBioscience 16-2012-83; RRID: AB_657692 CD150 167Er [TC15-12F12.2] Fluidigm 3167004B CD61 [2C9.G2] Biolegend 104302; RRID: AB_313079 Ly-6A/E (Sca-1)- 169Tm [D7] Fluidigm 3169015B pERK 1/2 (T202/Y204)- 171Yb [D13.14.4E] Fluidigm 3171010A CD117 (ckit)- 173Yb [2B8] Fluidigm 3173004B pStat3 (S727) [ab30647] Abcam ab86430; RRID: AB_779085 Pgc1a Abcam ab54481; RRID: AB_881987 Chemicals, Peptides, and Recombinant Proteins Romiplostim Kyowa Kirin n/a MitotrackerTM Green FM ThermoScientific M7514 TMRE Enzo 52309 CellROX ThermoScientific C10448 recombinant human Thpo (PEG-rHuMGDF) Kyowa Kirin n/a Filgrastim Kyowa Kirin n/a antimycin Sigma A8674 rotenone Abcam ab143145 oligomycin Abcam ab141829 FCCP Abcam ab120081 KPT-330 Cayman chemical 18127 murine recombinant SCF Peprotech 250-03 murine recombinant Thpo Peprotech 315-14 cisplatin (Cell-ID Cisplatin) Fluidigm 201198 Critical Commercial Assays CellTiter-Glo 2.0 Assay kit Promega G9241 MegaCult Stem cell Technologies 04960 MethoCult Stem cell Technologies M3234 LIVE/DEAD Viability/cytotoxicity Kit Life Technologies MP03224 SMARTer Ultra Low RNA Kit for Fluidigm C1 system Clonetech 634936 Illumina Nextera XT DNA Sample Preparation Kit Illumina FC-131-1096/FC-131-1002 C1 Single-cell Auto Prep Reagent kit for mRNA-seq Fluidigm 100-6201 Superscript VILO Invitrogen 11754250 RNeasy Mini Kit QIAGEN 74106 IntraPrep reagents Beckman Coulter IM2389 Maxpar antibody labeling kit Fluidigm 201300 MaxPar Fix I buffer Fluidigm 201065 Maxpar Cell Staining Buffer Fluidigm 201068 Cell-ID Intercalator-Ir Fluidigm 201192A Maxpar Fix and Perm Buffer Fluidigm 201057 (Continued on next page) Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data RNA-seq: Thrombopoietin metabolically primes hematopoietic stem cells to megakaryocyte lineage differentiation This paper GEO: GSE121001 Experimental Models: Organisms/Strains Mouse: Thpo / Toshio Suda Lab n/a Mouse: UBC-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J) Jackson laboratory 004353 Mouse: CD45.1 Jackson laboratory 002014 Mouse: Mito-Dendra2 (Gt(ROSA)26Sortm1.1(CAG-COX8A/ Dendra2)Dcc) Jackson laboratory 018385 Mouse: (Fucci) GMNN-mAG mice (B6.Cg-Tg(FucciS/G2/M)#474Bsi RIKEN RBRC02704 Oligonucleotides Taqman primer: Ppargc1a Thermo Fisher Scientific Mm01208835_m1 Taqman primer: Tfam Thermo Fisher Scientific Mm00447485_m1 Taqman primer: Nrf1 Thermo Fisher Scientific Mm01135606_m1 Taqman primer: Sdhb Thermo Fisher Scientific Mm00458272_m1 Taqman primer: Sdhc Thermo Fisher Scientific Mm00481172_m1 Taqman primer: Atp5a Thermo Fisher Scientific Mm00431960_m1 Taqman primer: Cox5a Thermo Fisher Scientific Mm00432638_m1 Taqman primer: Cyc1 Thermo Fisher Scientific Mm00470540_m1 Taqman primer: Ndufa13 Thermo Fisher Scientific Mm00445751_m1 Taqman primer: B2m Thermo Fisher Scientific Mm03003532_u1 Taqman primer: Bax Thermo Fisher Scientific Mm00432051_m1 Taqman primer: Bak1 Thermo Fisher Scientific Mm00432045_m1 Taqman primer: Bcl2 Thermo Fisher Scientific Mm00477631_m1 Taqman primer: Prdx1 Thermo Fisher Scientific Mm01621996_s1 Taqman primer: Prdx3 Thermo Fisher Scientific Mm00545848_m1 Taqman primer: Gpx1 Thermo Fisher Scientific Mm00656767_g1 Taqman primer: Gpx4 Thermo Fisher Scientific Mm00515041_m1 Taqman primer: Txn2 Thermo Fisher Scientific Mm00444931_m1 Software and Algorithms FlowJo 10.1 Tree Star n/a QuantStudioTM Design and Analysis Software 1.3.1 Thermo Fisher Scientific n/a Agilent Seahorse Wave Desktop Agilent n/a R R Foundation version 3.3.2 Rtsne package version 0.13 Cufflinks version 2.2.1 PANTHER release 20160715 GSEA Broad institute Version 2.0.13 NIS elements Nikon n/a ImageJ n/a Cytobank. n/a flowCore n/a flowViz n/a flowUtils n/a

    Staining:

    Article Title: Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD16/32 [93] eBioscience / Biolegend 14-0161 / 101320; RRID: AB_312800/AB_467132 CD16/32 [93], AF700 eBioscience 56-0161-82; RRID: AB_493994 CD4 [RM4-5], PerCp-Cy5.5 Biolegend 100540; RRID: AB_2563022 CD8a [53-6.7], PerCp-Cy5.5 Biolegend 100734; RRID: AB_893427 Gr-1 [RB6-8C5], PerCp-Cy5.5 Biolegend 108428; RRID: AB_893561 Mac-1 [M1/70], PerCp-Cy5.5 Biolegend 101228; RRID: AB_893233 B220 [RA3-6B2], PerCp-Cy5.5 Biolegend 103236; RRID: AB_893356 Ter-119 [TER-119], PerCp-Cy5.5 Biolegend 116228; RRID: AB_893638 c-Kit [2B8], APC-Cy7 Biolegend 105826; RRID: AB_1626280 Sca-1 [D7], PE-Cy7 Biolegend 108114; RIID: AB_493597 Sca-1 [D7], APC-Cy7 Biolegend 108126; RRID: AB_10639725 CD150 [TC15-12F12.2], APC Biolegend 115910; RRID: AB_493461 CD150 [TC15-12F12.2], PE Biolegend 115904; RRID: AB_313682 CD150 [TC15-12F12.2], PEcy7 Biolegend 115914; RRID: AB_439796 CD48 [HM48-1], AF700 Biolegend 103426; RRID: AB_10612754 CD45.1 [A20], PE Biolegend 110708; RRID: AB_313496 CD45.1 [A20], AF700 Biolegend 110724; RRID: AB_493732 CD45.2 [104], APC-Cy7 Biolegend 109824; RRID: AB_830788 CD8a [53-6.7], PE-Cy7 Biolegend 100722; RRID: AB_312760 Gr-1 [RB6-8C5], APC BD 553129; RRID: AB_2314614 Mac-1 [M1/70], APC Biolegend 101212; RRID: AB_312794 B220 [RA3-6B2], PECy7 BD 552772; RRID: AB_394458 c-Kit [2B8], BV421 Biolegend 105828; RRID: AB_10898120 Flt-3 [A2F10.1], APC eBioscience 17-1351-80; RRID: AB_10596653 Flt-3 [A2F10.1], PE Biolegend 135305; RRID: AB_1877217 CD41 [MWReg30], PE BD 558040; RRID: AB_397004 CD41 [MWReg30], APC Invitrogen 17-0411-82; RRID: AB_1603238 IL-7Ra [A7R34], PE eBioscience 12-1271-81; RRID: AB_465845 IL-7Ra [A7R34], BV510 Biolegend 135033; RRID: AB_2564576 Endoglin [MJ7/18] Biolegend 120410; RRID: AB_1027702 CD9 [KMC8], BV421 BD Horizon 564235; RRID: AB_395032 CD34 [RAM34], FITC eBioscience 11-034-85; RRID: AB_465020 Mpl [AMM2], Biotin IBL 10403 Ki67 [SolA15], PE eBioscience 12-5698-82; RRID: AB_11150954 AnnexinV, PE Life Technologies A35111 pSTAT3 (S727)[49] BD Phosflow 558557 GRIM19 [6E1BH7] Abcam ab110240; RRID: AB_303811 TOMM20 Abcam Ab186734; RRID: AB_2043078 pAMPKa (Thr172) [40H9] CST 2535; RRID: AB_331250 CD41-143Nd [MWreg30] Fluidigm 3143009B SOCS3 [516919] R&D MAB5696; RRID: AB_2193299 pStat5 (Y694)-147Sm [47] Fluidigm 3147012A SOCS1 [4H1] ThermoScientific 37-4100; RRID: AB_10890785 (Continued on next page) e1 Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER anti-biotin-150Nd Fluidigm 3150008B pAkt (S473) 152Sm [D9E] Fluidigm 3152005A pStat1 (Y701)- 153Eu [58D6] Fluidigm 3153003A CD48-154Sm [HM48-1] Fluidigm 3154004B p38 (T180/Y182)- 156Gd [D3F9] Fluidigm 3156002A pStat3 (Y705)- 158Gd [4] Fluidigm 3158005A pMAPKAPK2 (T334)- 159Tb [27B7] Fluidigm 3159010A pJak2 (Tyr1008) [D4A8] CST 8082S; RRID: AB_10949104 Tie2 [TEK4] Biolegend 124002; RRID: AB_961175 mTOR (phospho S2448) [EPR426(2)] Abcam ab109268; RRID: AB_297588 EPCR [eBio1560] eBioscience 16-2012-83; RRID: AB_657692 CD150 167Er [TC15-12F12.2] Fluidigm 3167004B CD61 [2C9.G2] Biolegend 104302; RRID: AB_313079 Ly-6A/E (Sca-1)- 169Tm [D7] Fluidigm 3169015B pERK 1/2 (T202/Y204)- 171Yb [D13.14.4E] Fluidigm 3171010A CD117 (ckit)- 173Yb [2B8] Fluidigm 3173004B pStat3 (S727) [ab30647] Abcam ab86430; RRID: AB_779085 Pgc1a Abcam ab54481; RRID: AB_881987 Chemicals, Peptides, and Recombinant Proteins Romiplostim Kyowa Kirin n/a MitotrackerTM Green FM ThermoScientific M7514 TMRE Enzo 52309 CellROX ThermoScientific C10448 recombinant human Thpo (PEG-rHuMGDF) Kyowa Kirin n/a Filgrastim Kyowa Kirin n/a antimycin Sigma A8674 rotenone Abcam ab143145 oligomycin Abcam ab141829 FCCP Abcam ab120081 KPT-330 Cayman chemical 18127 murine recombinant SCF Peprotech 250-03 murine recombinant Thpo Peprotech 315-14 cisplatin (Cell-ID Cisplatin) Fluidigm 201198 Critical Commercial Assays CellTiter-Glo 2.0 Assay kit Promega G9241 MegaCult Stem cell Technologies 04960 MethoCult Stem cell Technologies M3234 LIVE/DEAD Viability/cytotoxicity Kit Life Technologies MP03224 SMARTer Ultra Low RNA Kit for Fluidigm C1 system Clonetech 634936 Illumina Nextera XT DNA Sample Preparation Kit Illumina FC-131-1096/FC-131-1002 C1 Single-cell Auto Prep Reagent kit for mRNA-seq Fluidigm 100-6201 Superscript VILO Invitrogen 11754250 RNeasy Mini Kit QIAGEN 74106 IntraPrep reagents Beckman Coulter IM2389 Maxpar antibody labeling kit Fluidigm 201300 MaxPar Fix I buffer Fluidigm 201065 Maxpar Cell Staining Buffer Fluidigm 201068 Cell-ID Intercalator-Ir Fluidigm 201192A Maxpar Fix and Perm Buffer Fluidigm 201057 (Continued on next page) Cell Reports 25, 1772–1785.e1–e6, November 13, 2018 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data RNA-seq: Thrombopoietin metabolically primes hematopoietic stem cells to megakaryocyte lineage differentiation This paper GEO: GSE121001 Experimental Models: Organisms/Strains Mouse: Thpo / Toshio Suda Lab n/a Mouse: UBC-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J) Jackson laboratory 004353 Mouse: CD45.1 Jackson laboratory 002014 Mouse: Mito-Dendra2 (Gt(ROSA)26Sortm1.1(CAG-COX8A/ Dendra2)Dcc) Jackson laboratory 018385 Mouse: (Fucci) GMNN-mAG mice (B6.Cg-Tg(FucciS/G2/M)#474Bsi RIKEN RBRC02704 Oligonucleotides Taqman primer: Ppargc1a Thermo Fisher Scientific Mm01208835_m1 Taqman primer: Tfam Thermo Fisher Scientific Mm00447485_m1 Taqman primer: Nrf1 Thermo Fisher Scientific Mm01135606_m1 Taqman primer: Sdhb Thermo Fisher Scientific Mm00458272_m1 Taqman primer: Sdhc Thermo Fisher Scientific Mm00481172_m1 Taqman primer: Atp5a Thermo Fisher Scientific Mm00431960_m1 Taqman primer: Cox5a Thermo Fisher Scientific Mm00432638_m1 Taqman primer: Cyc1 Thermo Fisher Scientific Mm00470540_m1 Taqman primer: Ndufa13 Thermo Fisher Scientific Mm00445751_m1 Taqman primer: B2m Thermo Fisher Scientific Mm03003532_u1 Taqman primer: Bax Thermo Fisher Scientific Mm00432051_m1 Taqman primer: Bak1 Thermo Fisher Scientific Mm00432045_m1 Taqman primer: Bcl2 Thermo Fisher Scientific Mm00477631_m1 Taqman primer: Prdx1 Thermo Fisher Scientific Mm01621996_s1 Taqman primer: Prdx3 Thermo Fisher Scientific Mm00545848_m1 Taqman primer: Gpx1 Thermo Fisher Scientific Mm00656767_g1 Taqman primer: Gpx4 Thermo Fisher Scientific Mm00515041_m1 Taqman primer: Txn2 Thermo Fisher Scientific Mm00444931_m1 Software and Algorithms FlowJo 10.1 Tree Star n/a QuantStudioTM Design and Analysis Software 1.3.1 Thermo Fisher Scientific n/a Agilent Seahorse Wave Desktop Agilent n/a R R Foundation version 3.3.2 Rtsne package version 0.13 Cufflinks version 2.2.1 PANTHER release 20160715 GSEA Broad institute Version 2.0.13 NIS elements Nikon n/a ImageJ n/a Cytobank. n/a flowCore n/a flowViz n/a flowUtils n/a



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